Paleo-DNA Training Courses - Practical DNA Skills Training Course (Public)

This offering is approved as a Micro-credential by the Ministry of Colleges and Universities (MCU) of Ontario and is OSAP-MC eligible at Lakehead University.

To learn more about the provincial program and how to apply for OSAP for Micro-credentials, please visit Ontario.ca Micro-credentials from Ontario’s postsecondary schools.
For a list of frequently asked questions on OSAP for Micro-credentials, please visit Lakehead Student Central: Financing, Scholarships & Awards and Government Aid.
To learn more about OSAP for Micro-credentials, please visit Lakehead Student Central.

This two-week (nine business day) intensive laboratory-based training program is designed to teach learners the fundamentals of molecular techniques including DNA extraction, amplification (using PCR), sequencing, and interpretation. The course includes training in the latest DNA technologies, including multiplex PCR, real time PCR and use of the 3130xl automated sequencer. The laboratory sessions will have no more than eight learners with our lab instructors.

Laboratory 1: Laboratory Techniques
Laboratory 2: Sample Collection and Preparation
Laboratory 3: Gel Electrophoresis
Laboratory 4: Extraction Procedures
Laboratory 5: Purification Procedures
Laboratory 6: Quantification Procedures
Laboratory 7: Mitochondrial DNA PCR Preparation
Laboratory 8: Gel Electrophoresis
Laboratory 9: Pre-Sequence Purification
Laboratory 10: Sequencing PCR Preparation
Laboratory 11: Post-Sequencing Purification
Laboratory 12: STR Multiplex PCR Preparation
Laboratory 13: Tissue Sample Preparation
Laboratory 14: Computer Lab

The laboratory sessions are designed to follow the general process of the collection and preparation of a sample followed by the extraction and analysis of the DNA. The laboratories will cover the different types of samples that can be used for DNA analysis (buccal, hair, blood, bone) and the different preparation procedures required for each sample type. The participants will learn various DNA extraction methods applied to a variety of tissue types. Learners practice on their own DNA samples using PCR (polymerase chain reaction), Multiplex PCR, Real-time PCR, Sequencing, and fragment analysis. Other techniques covered in the lab sessions include gel (Agarose and polyacrylamide) and capillary electrophoresis, measuring, centrifugation and pipetting. The participants will analyze the control region of their own mitochondrial DNA, and STRs (short tandem repeats) within their nuclear DNA. One laboratory session is also dedicated to the computational aspects of analyzing DNA wherein each participant has their own computer and works through databases, websites and programs that are applicable to their analysis or the interpretation of their results.

A Non-Credit Transcript will be issued by Lakehead University and a Certificate of Participation will be issued by the Paleo-DNA Laboratory.

If you are looking for a good textbook to purchase before attending the Practical DNA Training Program, here is one we recommend:

Goodwin, William, et al. An Introduction to Forensic Genetics, 2nd Edition. Wiley-Blackwell, 2010.

Learners joining us from outside the city may wish to check out the following links for accommodations:

• Lakehead University Conference Services
• Thunder Bay Hostels

For more information, please contact the Paleo-DNA Laboratory at 807-343-8010, ext. 8616 or paleodna@lakeheadu.ca.